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#pombe

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Eishi Noguchi<p>My pombe (fission yeast) didn’t mate 😌<br>Preparing a different medium for mating, giving them some B vitamins 😉<br>Yeast needs vitamins, I also need vitamins 🤔</p><p><a href="https://masto.ai/tags/Pombe" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Pombe</span></a> <a href="https://masto.ai/tags/Yeast" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Yeast</span></a> <a href="https://masto.ai/tags/Science" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Science</span></a> <a href="https://masto.ai/tags/Experiment" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Experiment</span></a> <a href="https://masto.ai/tags/Vitamins" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Vitamins</span></a> <a href="https://masto.ai/tags/Health" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Health</span></a></p>
Eishi Noguchi<p>A beautiful Western blot that I did with new undergraduate students 🥳 </p><p>S. pombe nmt1 (81) promoter worked well to repress gene expression 🧐</p><p><a href="https://masto.ai/tags/Science" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Science</span></a> <a href="https://masto.ai/tags/WesterBlotting" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>WesterBlotting</span></a> <a href="https://masto.ai/tags/Protein" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Protein</span></a> <a href="https://masto.ai/tags/Pombe" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Pombe</span></a> <a href="https://masto.ai/tags/Promoter" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Promoter</span></a> <a href="https://masto.ai/tags/Photography" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Photography</span></a></p>
Eishi Noguchi<p>I spent the day running experiments with yeast (S. pombe) instead of sitting in front of a computer 😊<br>I wondered how much yeast (S. cerevisiae) is in this cloudy IPA, looks like way more than I used in my lab today 🤔</p><p><a href="https://masto.ai/tags/Beer" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Beer</span></a> <a href="https://masto.ai/tags/IPA" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>IPA</span></a> <a href="https://masto.ai/tags/Yeast" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Yeast</span></a> <a href="https://masto.ai/tags/Pombe" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Pombe</span></a> <a href="https://masto.ai/tags/Science" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Science</span></a> <a href="https://masto.ai/tags/Photography" class="mention hashtag" rel="nofollow noopener noreferrer" target="_blank">#<span>Photography</span></a></p>

Tomorrow is the deadline, the office staff told me that the NIH grant application system has been a bit unstable, my brain is also a bit unstable because of sleep deprivation, so I made some agar plates to clear my mind, it seemed to help 🤔

#ThursdayThoughts

While running last-minute experiments to support my proposal, a student in a neighboring lab said he’d never seen me “doing science” before. I'm sure he meant “doing experiments,” but I do feel I'm “doing science” when I'm doing experiments, rather than working (on science) at a computer🤔

Making some agar growth medium for facilitating fission yeast mating😊
They have two mating types: h- and h+ 😉
Malt extract in the medium 😊
Fission yeast - Schizosaccharomyces pombe 😉

I just made a successful pollination for our pumpkin (I think), now fission yeast’s turn 😊